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Worthington-Biochem代理商及品牌介绍( Worthington特约代理)-上海起发

更新时间:2026-03-23点击次数:23

一、公司介绍

自1947年创立以来,Worthington-Biochem始终致力于为生命科学领域提供高纯度酶制剂及相关生化试剂。作为酶学领域的优秀者,公司以“推动科研突破与生物工艺革新"为核心使命,凭借深厚的技术积淀和持续的创新能力,成为100多个国家科研机构、生物制药企业及诊断实验室的合作伙伴。  

从经典的细胞分离酶到前沿的无动物源产品线,从分子生物学级核酸酶到定制化生物工艺解决方案,Worthington-Biochem的产品覆盖生命科学研究的全链条。其技术优势源于对酶活性、稳定性和安全性的追求,以及对客户需求快速响应的专业服务团队。  


📌 下图为"上海起发"作为其授权代理的授权书

Worthington-biochem.jpg

二、核心优势

1. 78年技术积淀,专注酶学领域  

   • 深耕酶纯化技术,从动物组织、植物提取到微生物发酵,掌握酶来源筛选与活性优化方案。  

   • 产品线覆盖7大类、数百种酶制剂,满足从基础研究到大规模生产的多样化需求。  

2. 科学验证,实验可重复性保障  

   • 每批次产品提供详细的生化参数(如比活、蛋白含量、内毒素水平),确保实验结果稳定可靠。  

   • 与哈佛医学院、中科院等机构合作,累计支持超5600篇科研文献发表。  

3. 严格的质量管理体系  

   • 生产流程,从原料采购到成品包装全程可追溯。  

   • 无动物源(Animal Free)系列产品通过GMP兼容性验证,消除BSE/TSE及哺乳动物病毒风险。  

4. 定制化服务能力  

   • 针对生物制药、疫苗生产等场景,提供酶活性优化、配方调整等专业支持。  

   • 技术团队提供实验方案设计、数据分析等全流程服务。  


三、热门产品介绍

1. 胶原蛋白酶(Collagenase)

• 产品特点:  

  • 提供7种亚型(Type I-IV、Purified等),适配脂肪、肝脏、心脏、肿瘤等不同组织解离需求。  

  • 无动物源配方(STEMxyme®系列)支持干细胞研究与临床转化。  

• 存储条件:-20℃避光保存,避免反复冻融。  

• 工作原理:特异性降解胶原蛋白中的Pro-Y-Gly-Pro序列,协同中性蛋白酶、透明质酸酶分解细胞间基质。  

• 使用方法:根据组织类型调整酶浓度(通常1-2 mg/mL)与孵育时间(30分钟至2小时),配合机械剪切优化解离效率。  

2. 脱氧核糖核酸酶I(DNase I)

• 产品特点:  

  • 分为研究级(DPRF/DPRFS)与重组级(DR1/DR1S),RNase和蛋白酶污染低于检测限。  

  • 适用于RNA提取、RT-PCR前处理及生物制药工艺中的DNA清除。  

• 存储条件:溶液型-20℃,冻干粉2-8℃。  

• 工作原理:非特异性切割DNA的3',5'-磷酸二酯键,优先消化基因组DNA。  

• 使用方法:在RNA样品中加入1-2 U/μL DNase I,37℃孵育30分钟,通过酚氯仿抽提去除酶残留。  

3. 弹性蛋白酶(Elastase)

• 产品特点:  

  • 纯化自猪胰腺,高效水解弹性蛋白,适用于肺细胞、肿瘤微环境研究。  

  • 低蛋白酶活性设计,减少对细胞膜蛋白的损伤。  

• 存储条件:冻干粉2-8℃,复溶后-20℃短期保存。  

• 工作原理:特异性切割弹性蛋白的Ala-Ala键,保留胶原蛋白结构。  

• 使用方法:与胶原酶联用(比例1:5),37℃消化肺组织块1-3小时。  

4. 木瓜蛋白酶(Papain)

• 产品特点:  

  • 从番木瓜乳胶中提取,温和解离神经组织与胚胎样本。  

  • 支持流式细胞术分选前的细胞预处理。  

• 存储条件:冻干粉2-8℃,复溶后分装冻存。  

• 工作原理:水解细胞外基质中的肽键,保留细胞表面抗原完整性。  

• 使用方法:10-20 mg/mL浓度,37℃孵育1-2小时,加入半胱氨酸激活酶活。  

5. 胰蛋白酶(Trypsin)

• 产品特点:  

  • TPCK处理型减少自溶,适用于蛋白质组学与测序样本制备。  

  • 无外泌体残留配方,保障单细胞测序数据准确性。  

• 存储条件:冻干粉2-8℃,溶液型-20℃。  

• 工作原理:切割赖氨酸与精氨酸的羧基端,释放单细胞悬液。  

• 使用方法:0.25%浓度(含0.02% EDTA),37℃消化10-30分钟,胰酶抑制剂终止反应。  


四、解决实验中的核心问题

1. 细胞分离效率低  

   • 痛点:传统酶制剂活性不稳定,导致组织解离不全。  

   • 解决方案:多酶协同配方(如胶原酶+中性蛋白酶)精准匹配组织类型,提升细胞得率。  

2. DNA污染影响分子实验  

   • 痛点:质粒构建或RNA提取中基因组DNA残留。  

   • 解决方案:DNase I(DPRF级)在无RNase环境下清除DNA,保障实验纯度。  

3. 动物源成分限制临床转化  

   • 痛点:BSE/TSE风险阻碍干细胞治疗与疫苗申报。  

   • 解决方案:无动物源胶原酶(CLSAFA)、AF Trypsin等产品符合GMP要求。  

4. 批次间差异干扰结果  

   • 痛点:不同批次酶活性波动导致实验可重复性差。  

   • 解决方案:每批次提供活性证书(COA),支持客户定制质检方案。  


五、目标客户群体

• 科研机构:高校、研究所的细胞生物学、分子生物学实验室。  

• 生物制药企业:疫苗生产、抗体药物开发、基因治疗研发部门。  

• 诊断实验室:需要高特异性酶联免疫检测(ELISA)的技术平台。  

• 教学机构:医学院、生物工程专业的实验教学与技能培训。  


六、购买常见问题与解答

Q1:酶制品的活性单位如何定义?  

A:活性单位基于特定底物反应体系(如胶原酶降解牛跟腱胶原的速率),确保跨批次可比性。  

Q2:无动物源产品是否适用于大规模生产?  

A:是的,曲霉发酵来源的DNase I、植物源蛋白酶等已通过中试验证,支持GMP批次生产。  

Q3:如何选择适合的胶原酶亚型?  

A:参考组织类型——肝脏优选Type IV(低胰酶活性),肿瘤组织推荐Type I+II组合。  

Q4:产品运输是否全程冷链?  

A:干粉产品常温运输,液体酶采用干冰冷链,确保活性稳定。  

Q5:能否提供小样试用?  

A:支持免费样品申请,需提供实验方案以便推荐适配产品。  


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更多产品信息,请联系中国区域代理商:上海起发实验试剂有限公司

如果您对任意产品或其他品牌感兴趣,可通过“在线留言"功能留下您的需求

(注:本文内容基于品牌公开资料及行业常规信息整理,具体以品牌信息文档为准。)


🔖卖产品

货号

品名

规格

品牌

LS004176

Collagenase, Type 2 (CLS-2)2型胶原酶

1gm

Worthington Biochemical

LS004196

Collagenase Type 1

1gm

Worthington Biochemical

LS004188

Collagenase, Type 4

1gm

Worthington Biochemical

LS004194

Collagenase Type 1 (CLS-1)

100mg

Worthington Biochemical

LS004174

Collagenase, Type 2

100mg

Worthington Biochemical

LS004186

Collagenase, Type 4

100mg

Worthington Biochemical

LS004182

Collagese, Type 3

1gm

Worthington Biochemical

ls003126

Papain, Suspension

100mg

Worthington Biochemical

LK003150

Papain Dissociation System

1bx

Worthington Biochemical

LS003119

Papain, Lyophilized

100mg

Worthington Biochemical

LS004180

Collagenase, Type 3

100mg

Worthington Biochemical

LS003740

Trypsin, TPCK Treated

100mg

Worthington Biochemical

LS002139

Deoxyribonuclease I

100mg

Worthington Biochemical

LK003178

PDS Kit Papain Vial

5vi

Worthington Biochemical

LS002292

Elastase Lyophilized

100mg

Worthington Biochemical

LS002279

Elastase, Suspension (ES)

100mg

Worthington Biochemical

LS004177

Collagese, Type 2

5gm

Worthington Biochemical

LS004189

Collagenase, Type 4

5 gm

Worthington Biochemical

LS002006

Deoxyribonuclease I

20mg

Worthington Biochemical

LS003926

Phosphodiesterase I

100un

Worthington Biochemical

LK003176

PDS Kit Papain Vial (PAP2)

1 vi

Worthington Biochemical

LS002007

Deoxyribonuclease I

100mg

Worthington Biochemical

LS05006

Reverse Transcriptase, Recombinant, HIV

5x200 un

Worthington Biochemical

LS002140

Deoxyribonuclease I

1 gm

Worthington Biochemical

LK003170

DNase Vial (D2) (D2

1 vi

Worthington Biochemical

LK003182

PDS Kit Inhibitor Vial (OI-BSA)

1 vi

Worthington Biochemical

LS002594

Hyaluronidase

50ku

Worthington Biochemical

LS002290

Elastase, Lyophilized

25mg

Worthington Biochemical

LS004106

STEMxyme® 1, Collagenase/Neutral Protease (Dispase®), Animal Free

50mg

Worthington Biochemical

LS02100

Neutral Protease (Dispase), Purified

10mg

Worthington Biochemical

LS003570

Trypsin Inhibitor, Soybean, Purified

100mg

Worthington Biochemical

LS003608

Protease, S. Aureus (Endoproteinase Glu-C)

1mg

Worthington Biochemical

LS002274

Elastase, Suspension

25mg

Worthington Biochemical

LS003127

Papain, Suspension

1 gm

Worthington Biochemical

LS003086

Trypsin Inhbitor, Ovomucoid

2 gm

Worthington Biochemical

LS005305

Carboxypeptidase B

5mg

Worthington Biochemical

LS004212

Collagenase, Type 4, Filtered Store at 2-8°C.

5x50mg

Worthington Biochemical

LS003118

Papain, Lyophilized

25mg

Worthington Biochemical

LS004200

Collagenase, Type 1

Bulk

Worthington Biochemical

LS004179

Collagese, Type 2

Bulk

Worthington Biochemical

LS004197

Collagenase Type 1

5 gm

Worthington Biochemical

LS004798

Nuclease, Micrococcal

45 ku

Worthington Biochemical

LS003120

Papain, Lyophilized

1 gm

Worthington Biochemical

LS004191

Collagenase, Type 4

500g

Worthington Biochemical

LS006344

Deoxyribonuclease I, RNase & Protease Free, Solution

500 un

Worthington Biochemical

LS001070

Alcohol Dehydrogese, Lyophilized

1gm

Worthington Biochemical

LS002592

Hyaluronidase

300ku

Worthington Biochemical

LS02104

Neutral Protease (Dispase), Purified

50mg

Worthington Biochemical

LS003124

Papain, Suspension

25mg

Worthington Biochemical

LS003571

Trypsin Inhibitor, Soybean, Purified

1 gm

Worthington Biochemical

LS004520

Galactose Oxidase

150 un

Worthington Biochemical

LS004522

Galactose Oxidase

450 un

Worthington Biochemical

LS002138

Deoxyribonuclease I

25mg

Worthington Biochemical

LS003793

Polyphenol Oxidase (Tyrosinase)

500 ku

Worthington Biochemical

LS004524

Galactose Oxidase

1 ku

Worthington Biochemical

LK003163

Papain Dissociation System, Without EBSS

3 bx

Worthington Biochemical

LS003605

Protease, S. Aureus (Endoproteinase Glu-C)

5mg

Worthington Biochemical

LS003744

Trypsin, TPCK Treated

1 gm

Worthington Biochemical

LS005275

Collagenase, Purified

4ku

Worthington Biochemical

LS006323(500ku)

Deoxyribonuclease I, Recombint, Animal Free, Bioprocess Grade

500ku

Worthington Biochemical

LS02111

Neutral Protease, Partially Purified

5gm

Worthington Biochemical

LS003602

Phosphodiesterase II

25un

Worthington Biochemical

LS006363

Elastase, Purified

5mg

Worthington Biochemical

LS005282

Collagenase, Type 5

1gm

Worthington Biochemical

LS02122

Trypsin, Modified, SequENZ® Sequencing Grade

4x100ug

Worthington Biochemical

LS003741

Trypsin, TPCK Treated (TRTPCK)

500mg

Worthington Biochemical

LS05003

Reverse Transcriptase, Recombinant, HIV

200 un

Worthington Biochemical

LS006123

Phosphatase, Alkaline, Purified

25mg

Worthington Biochemical

LS002933

Lysozyme, Purified, Salt Free

5 gm

Worthington Biochemical

LS003587

Trypsin Inhibitor, Soybean

1 gm

Worthington Biochemical

LS01492

Ribonuclease T1, Chromatographically Purif., Lyophilized

2500ku

Worthington Biochemical

LK003172

DNase Vial (D2)

5 vi

Worthington Biochemical

LS002106

Deoxyribonucleic Acid, Calf Thymus

1 gm

Worthington Biochemical

LS003887

Urease

10 gm

Worthington Biochemical

LK003153

Papain Dissociation System

3bx

Worthington Biochemical

LS001263

Carbonic Anhydrase

250mg

Worthington Biochemical

LS006323

Deoxyribonuclease I, Recombinant, Animal Free, Bioprocess Grade

500ku

Worthington Biochemical

LS001656

Collagen

10 gm

Worthington Biochemical

LS001334

Chymotrypsin, Alpha, 1X

10 gm

Worthington Biochemical

LS005477

Hyaluronidase, Purified

5ku

Worthington Biochemical

LS004081

Phosphatase, Alkaline

10mg

Worthington Biochemical

LS004780

Neuraminidase

10mg

Worthington Biochemical

LS003589

Trypsin Inhibitor, Soybean

10gm

Worthington Biochemical

LS004154

Collagenase, Type A, Animal Free

1gm

Worthington Biochemical

LS001141

Phosphatase, Acid

1gm

Worthington Biochemical

LS004185

Collagenase, Type 3

500g

Worthington Biochemical

LK003300

Neonatal Cardiomyocyte Isolation System

1 kt

Worthington Biochemical

LS003750

Trypsin, TPCK-Treated, Irradiated

100mg

Worthington Biochemical

LS006331

Deoxyribonuclease I, Ribonuclease & Protease Free

2500un

Worthington Biochemical

LK003188

PDS Kit, EBSS Via

1vi

Worthington Biochemical

LS003981

Glucose-6-Phosphate Dehydrogenase, Lyophilized

1 ku

Worthington Biochemical

LS005273

Collagenase, Purified

10 ku

Worthington Biochemical

LS003707

Trypsin, Purified

1 gm

Worthington Biochemical

LS01502

Ribonuclease T2, Recombinant

250ku

Worthington Biochemical

LS006355

Deoxyribonuclease I, Recombinant, Solution

5x2 ku

Worthington Biochemical

LS02123

Trypsin, Modified, Sequencing Grade

1mg

Worthington Biochemical

LS004210

Collagenase, Type 4, Filtered

50mg

Worthington Biochemical

LS002512

Hexokinase, Lyophilized

10 ku

Worthington Biochemical

LS006308

Amino Acid Oxidase, D-

25mg

Worthington Biochemical

LS004326-50ku

Diaphorase

50ku

Worthington Biochemical