complementtech/Medicago/emfret/complementtech厂家答疑与实验指导

●问题1

货号：A137

品名：Factor H

品牌：complementtech

原文：

Q:May I ask if different batches of this product (A137) can be mixed and if this antibody has been tested for endotoxins?

A:Sorry for the delay.

Yes, you can mix the lots.  You will have to test, etc for activity.  If endotoxin tests were performed it would be listed on the applicable CoA.  If it is not listed then we did not test for endotoxins.

译文：

问：请问该产品（A137）的不同批次能否混合使用？该抗体是否做过内毒素检测？

答：抱歉回复延迟。

不同批次的产品可以混合使用，你需要对混合后的产品进行活性等相关检测。如果产品做过内毒素检测，相关结果会在对应的分析报告中列出；若报告中未提及，则说明该产品未进行内毒素检测。

●问题2

货号：10-0027-1

品名：Protein L Ligand Leakage ELISA

品牌：Medicago

原文：

Q:Customer feedback: Why has the expiration date of protein L reference （lot：285004） from the same batch been extended from 2025-10 to 2026-8 and 2027-8? Have you retested the protein L reference? If so, could you provide relevant statements and data?

A:Yes, we have extended the expiration date for the entire kit, including the references. There is no better proof than these certificates that the references have the same expiration date as the kit.

译文：

问：客户反馈：同批次的蛋白L标准品（批号：285004）有效期为何从2025年10月分别延长至2026年8月和2027年8月？贵司是否对该蛋白L标准品重新进行了检测？若有，烦请提供相关说明及检测数据。

答：我们已延长了整套试剂盒（包括参考品在内）的有效期。这些证书就是参考品与试剂盒有效期一致的最有力证明。

●问题3

货号：M050-1

品名：GPIbbeta (CD42c)Xia.C3 Rat IgG2a FITC; 300 tests

品牌：emfret

原文：

Q:The customer would like to inquire about the concentration of this antibody (M050-1)

A:Thank you for your inquiry.

We titrate the fluorescence-conjugated antibodies in relation to our negative controls (P190-X) so that optimal results are achieved. The ab concentration is not determined afterwards.

I am sorry to have no other information.

译文：

问：客户想咨询该抗体（M050-1）的浓度是多少？

答：感谢你的咨询。

我们以P190-X阴性对照为参照，对荧光标记抗体进行滴定，以确保实验能得到最佳结果，后续并未测定该抗体的浓度。

很抱歉，目前没有更多相关信息可以提供。

●问题4

货号：A137

品名：Factor H

品牌：complementtech

原文：

Q:Hello, may I ask how the extinction coefficient of A137 was obtained? Was it measured experimentally or by other means? It differs quite significantly from the theoretical extinction coefficient based on the sequence. Thank you.

A:Hi Jane,

We have determined FH concentration spectrophotometrically using an extinction coefficient A280 nm (1% solution) of 12.4 (Ref#1). Dr. MK. Pangburn has extensively worked on Factor H. Based on the explanation given by Dr. MK Pangburn in his paper here is the summary: Extinction coefficients reported for factor H have been reported ranging from 9.0 to 19.5 (Ref#1 to 4). The calculated extinction coefficient from the sequence (Ref#4) that has been corrected for the large amount of polysaccharide in factor H is 13.2. Despite these differences, which are typical for a widely studied protein, the value of 12.4 is in the middle of the reported range and is closest to the calculated value after correction for the high content of polysaccharide.  

References

Pangburn, M. K., and H. J. Mu¨ller-Eberhard. 1983. Kinetic and thermodynamic analysis of the control of C3b by the complement regulatory proteins factors H and I. Biochemistry 22: 178–185).

Sim, R. B., and R. G. Discipio. 1982. Purification and structural studies on the complement system control protein IH (factor H). Biochem. J. 205: 285–293.

Nagaki, K., K. Iida, M. Okubo, and S. Inai. 1978. Reaction mechanims of 1H globulin. Int. Arch. Allergy Appl. Immunol. 57: 221–232.

Hakobyan, S., C. L. Harris, A. Tortajada, J. E. Goicochea de, A. Garcia-Layana, P. Fernandez-Robredo, C. S. Rodriguez de, and B. P. Morgan. 2008. Measurement of factor H variants in plasma using variant-specific monoclonal antibodies: application to assessing risk of age-related macular degeneration. Invest. Ophthalmol. Vis. Sci. 49: 1983–1990.

译文：

问：您好，请问A137的消光系数是如何确定的？是通过实验测定的，还是通过其他方法得出的？该数值与根据序列计算的理论消光系数存在较大差异，谢谢。

答：你好，简：

我们采用分光光度法，以12.4的280nm摩尔消光系数（1%溶液，参考文献1）测定了补体因子H（FH）的浓度。MK·潘伯恩博士对补体因子H有深入的研究，根据其论文中的解释，现总结如下：已有文献报道的补体因子H消光系数范围为9.0至19.5（参考文献1至4）；通过氨基酸序列计算，并针对补体因子H中大量的多糖成分进行校正后，得到的消光系数为13.2（参考文献4）。对于一种被广泛研究的蛋白质而言，出现上述消光系数的差异是正常现象，而12.4这一数值处于已报道的范围中间，且与经多糖高含量校正后的计算值最为接近。

参考文献

潘伯恩·M·K、米勒-埃伯哈德·H·J，1983年，补体调节蛋白H因子和I因子调控C3b的动力学与热力学分析，《生物化学》，22卷：178-185页

西姆·R·B、迪西皮奥·R·G，1982年，补体系统调节蛋白IH（H因子）的纯化与结构研究，《生物化学杂志》，205卷：285-293页

长胁·K、饭田·K、大久保·M、稻井·S，1978年，1H球蛋白的反应机制，《国际过敏反应与应用免疫学文献》，57卷：221-232页

哈科布扬·S、哈里斯·C·L、托尔塔哈达·A等，2008年，利用变异型特异性单克隆抗体检测血浆中补体因子H变异体及其在年龄相关性黄斑变性风险评估中的应用，《眼科研究与视光科学》，49卷：1983-1990页

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