Worthington-biochem美国 2026年终端采购售价清单-上海起发

发布时间：2026/4/16

点击次数：11

1. 关于Worthington Biochemical

Worthington Biochemical是一家在酶学领域拥有深厚积淀的专业公司。其核心业务是提供从动物组织、植物或微生物中提取、纯化的多种酶类产品。公司尤其以提供用于组织解离和细胞分离的胶原酶、胰蛋白酶、中性蛋白酶等系列产品而广为人知。此外，其产品线还涵盖核酸酶、磷酸酶、蛋白酶抑制剂等多种用于分子生物学和生化研究的工具酶。

公司拥有专业的研发和生产团队，专注于酶的生产工艺优化和质量控制。其生产流程注重从原料筛选、提取纯化到最终配比的多个环节，力求保证酶制剂的纯度、比活性和批次间的一致性。Worthington的产品在细胞培养的原代细胞分离、组织工程、生物大分子纯化与修饰等研究领域均有应用。

2. 核心优势

在组织解离酶领域的专业积累

Worthington在胶原酶、胰蛋白酶、中性蛋白酶等组织解离酶的生产和特性研究方面有长期的经验。公司提供不同纯度和特异性级别的多种胶原酶（如I型、II型、IV型等）以及多种配方的胰蛋白酶，以满足从不同组织（如肝脏、心脏、肿瘤、脂肪等）中分离高活力原代细胞的特定需求。

酶制剂的纯度与活性控制

公司注重其酶产品的纯度。通过层析等多种纯化手段，Worthington能够提供多种高纯度的酶制品，例如不含非特异性核酸酶活性的蛋白酶制品，这对于需要避免核酸降解的精细实验尤为重要。同时，其产品通常提供明确的比活性单位，方便研究者精确计算用量。

面向特定应用的定制化酶产品

除了标准产品，Worthington还能够根据用户的研究需求，提供特定配方、特定比例的混合酶制剂。例如，为特定的组织类型优化胶原酶、中性蛋白酶和弹性蛋白酶的混合比例，以提高特定原代细胞的分离得率和活力。

3. 解决实验中的常见问题

在生命科学研究中，特别是涉及原代细胞培养、组织处理和生物分子操作的实验中，研究者常面临以下挑战，Worthington的酶产品旨在为此提供解决方案：

原代细胞分离得率低、活性差

从复杂组织中分离具有高活力和功能性的原代细胞是一项有挑战性的工作。选择合适的解离酶是成功的关键。Worthington提供的多种类型和级别的胶原酶、胰蛋白酶，以及中性蛋白酶和弹性蛋白酶，允许研究者根据目标组织的细胞外基质组成，筛选或定制最合适的酶组合，以温和、有效地释放细胞，并尽可能保持细胞膜表面受体和功能的完整性。

酶解过程中细胞损伤严重

不合适的酶解条件（如酶浓度过高、时间过长、酶制剂中含有杂酶活性）容易导致分离出的细胞损伤或死亡。Worthington的高纯度酶制剂旨在减少非特异性水解作用，其产品附带详细的使用指南和建议起始条件，有助于研究者优化解离方案，降低细胞损伤。

分子生物学实验中存在核酸酶污染

在使用蛋白酶K等进行蛋白消化，或使用其他蛋白酶进行样品处理时，如果酶制品中含有痕量的核酸酶（如RNase、DNase）活性，可能会严重降解样品中的核酸。Worthington提供的分子生物学级蛋白酶（如经纯化去除核酸酶活性的蛋白酶），旨在避免这类污染，保护珍贵的核酸样本。

蛋白样品制备中的蛋白降解问题

在细胞或组织裂解过程中，内源性蛋白酶活性可能导致目标蛋白的降解。Worthington提供多种广谱的或特异性的蛋白酶抑制剂混合物，可有效抑制丝氨酸蛋白酶、半胱氨酸蛋白酶、天冬氨酸蛋白酶、金属蛋白酶等多种蛋白酶活性，有助于在蛋白提取和纯化过程中维持蛋白的稳定性。

酶活性测定需要高纯度底物或工具酶

在进行酶动力学研究或生化分析时，需要高纯度和已知比活性的工具酶。Worthington提供多种纯化的工具酶，如用于糖原磷酸化酶研究的磷酸化酶激酶、磷酸化酶磷酸酶等，为深入的酶学研究提供可靠材料。

4. 常见疑问与解答

Q1：如何为我的组织类型选择合适的胶原酶？

A1：选择胶原酶主要取决于组织中胶原蛋白的类型和丰度。Worthington提供I型、II型、IV型等多种胶原酶，其底物特异性有所不同。例如，I型胶原酶对天然I型胶原的活性较高，常用于肝、肺、心脏等组织的解离；II型胶原酶对天然II型胶原活性较高，更常用于软骨、脂肪等组织。建议查阅文献中类似组织的使用经验，或联系技术支持获取建议。通常，进行预实验筛选是必要的。

Q2：产品说明中的“比活性"单位是什么意思？如何计算用量？

A2：比活性通常指每毫克蛋白质所含的酶活性单位（如U/mg）。它是衡量酶制剂纯度的一个重要指标。计算用量时，您需要根据您希望达到的最终工作浓度（U/mL）和反应体积，结合产品的比活性和浓度（mg/mL）进行换算。产品说明书通常会提供应用推荐和计算示例。

Q3：胶原酶和胰蛋白酶可以混合使用吗？

A3：可以。在实际组织解离中，经常将胶原酶与中性蛋白酶或胰蛋白酶等结合使用，以更有效地分解细胞外基质中的多种成分。Worthington也提供预混合的酶制剂。混合比例需要根据组织类型和实验目标进行优化。公司网站或技术支持可能提供一些常见组织（如肿瘤、胰岛）的参考方案。

Q4：酶的储存条件是什么？复溶后能保存多久？

A4：绝大多数酶制剂为冻干粉形式，应在-20°C或更低温干燥保存。复溶时建议使用说明书推荐的缓冲液，并轻柔操作以避免泡沫产生导致变性。复溶后的酶溶液通常不稳定，建议分装后立即在-20°C或-80°C冷冻保存，并尽快使用。具体的保存条件和稳定期请务必遵循每个产品的说明书。

Q5：如果我对标准产品不满意，可以提供定制服务吗？

A5：可以联系Worthington的技术服务部门咨询定制服务的可行性。公司可以根据用户的具体需求，调整酶的纯度、提供特定比例的混合酶、或进行特殊包装。定制服务的价格、最小订单量和交付时间需具体商议。

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特别告知：为保证产品质量与良好性能，所以在购买相应产品的同时我司会收取一定量的运输费用！！！本次报价有效时间为2026年4月15日---2026年12月20日。

（*此为部分产品价格,完整价格请下载文件)

货号	品名	规格	价格	品牌
1235-01	Celase® GMP Collagenase Blend	1vi	16720	Worthington-biochem
1235-PKG	Celase® GMP Collagenase Blend, Special Packaging	1ea	1600	Worthington-biochem
LK002060	Hepatocyte Isolation System	1bx	7560	Worthington-biochem
LK002064	Hank's Balanced Salt Solution 10X (HBSS10)	1ea	1470	Worthington-biochem
LK002066	Collagenase/Elastase Vial (CLSH)	1vi	896	Worthington-biochem
LK002067	Collagenase/Elastase Vial (CLSH)	5vi	3780	Worthington-biochem
LK002069	Sodium bicarbonate, 7.5%, (NAH)	1ea	532	Worthington-biochem
LK002070	0.15m, MOPS Buffer, (MOPS)	1ea	616	Worthington-biochem
LK003150	Papain Dissociation System	1bx	4340	Worthington-biochem
LK003153	Papain Dissociation System	3bx	11340	Worthington-biochem
LK003160	Papain Dissociation System, Without EBSS	1bx	3920	Worthington-biochem
LK003163	Papain Dissociation System, Without EBSS	3bx	10500	Worthington-biochem
LK003170	DNase Vial (D2)	1vi	420	Worthington-biochem
LK003172	DNase Vial (D2)	5vi	1372	Worthington-biochem
LK003176	PDS Kit, Papain Vial	1vi	434	Worthington-biochem
LK003178	PDS Kit, Papain Vial	5vi	1456	Worthington-biochem
LK003182	PDS Kit, Inhibitor Vial	1vi	1260	Worthington-biochem
LK003188	PDS Kit, EBSS Vial	1vi	896	Worthington-biochem
LK003200	Cell Isolation Optimizing System	1bx	9100	Worthington-biochem
LK003210	HBSS Solution	1ea	952	Worthington-biochem
LK003220	Trypsin Vial, NCIS	1vi	238	Worthington-biochem
LK003225	Trypsin Vial, NCIS	5vi	770	Worthington-biochem
LK003230	Inhibitor Vial, NCIS	1vi	266	Worthington-biochem
LK003235	Inhibitor Vial, NCIS	5vi	770	Worthington-biochem
LK003240	Collagenase Vial, NCIS	1vi	518	Worthington-biochem
LK003245	Collagenase Vial, NCIS	5vi	2128	Worthington-biochem
LK003250	L-15 Media Powder (L15NK)	1ea	476	Worthington-biochem
LK003265	Cell Strainers (Falcon)	5ea	756	Worthington-biochem
LK003300	Neonatal Cardiomyocyte Isolation System	1kt	5180	Worthington-biochem
LK003303	Neonatal Cardiomyocyte Isolation System	3kt	13160	Worthington-biochem
LS000150	Lactoperoxidase	10mg	1316	Worthington-biochem
LS000151	Lactoperoxidase	50mg	5250	Worthington-biochem
LS000152	Lactoperoxidase	Bulk	询价	Worthington-biochem
LS000290	Albumin, Nuclease-Free	100mg	868	Worthington-biochem
LS000291	Albumin, Nuclease-Free	5x100mg	3920	Worthington-biochem
LS000292	Albumin, Nuclease-Free	Bulk	询价	Worthington-biochem
LS001041	Actin	1mg	784	Worthington-biochem
LS001043	Actin	Bulk	询价	Worthington-biochem
LS001045	Actin	5mg	2940	Worthington-biochem
LS001069	Alcohol Dehydrogenase, Lyophilized	100mg	1610	Worthington-biochem
LS001070	Alcohol Dehydrogenase, Lyophilized	1gm	12040	Worthington-biochem
LS001071	Alcohol Dehydrogenase, Lyophilized	Bulk	询价	Worthington-biochem
LS001089	Alcohol Dehydrogenase, Suspension	Bulk	询价	Worthington-biochem
LS001123	Aldolase, Suspension	100mg	2100	Worthington-biochem
LS001125	Aldolase, Suspension	Bulk	询价	Worthington-biochem
LS001128	Aldolase, Lyophilized	Bulk	询价	Worthington-biochem
LS001130	Aldolase, Lyophilized	100mg	2590	Worthington-biochem
LS001141	Phosphatase, Acid	1gm	1820	Worthington-biochem
LS001144	Phosphatase, Acid	Bulk	询价	Worthington-biochem
LS001260	Carbonic Anhydrase	50mg	1820	Worthington-biochem
LS001263	Carbonic Anhydrase	250mg	8190	Worthington-biochem
LS001265	Carbonic Anhydrase	Bulk	询价	Worthington-biochem
LS001332	Chymotrypsin, Alpha, 1X	Bulk	询价	Worthington-biochem
LS001333	Chymotrypsin, Alpha, 1X	1gm	560	Worthington-biochem
LS001334	Chymotrypsin, Alpha, 1X	10gm	3290	Worthington-biochem
LS001430	Chymotrypsin, Alpha, TLCK Treated	25mg	462	Worthington-biochem
LS001432	Chymotrypsin, Alpha, TLCK Treated	100mg	1120	Worthington-biochem
LS001434	Chymotrypsin, Alpha, TLCK Treated	1gm	8680	Worthington-biochem
LS001438	Chymotrypsin, Alpha, TLCK Treated	Bulk	询价	Worthington-biochem
LS001448	Chymotrypsin, Alpha, 3X	250mg	644	Worthington-biochem
LS001450	Chymotrypsin, Alpha, 3X	1gm	1862	Worthington-biochem
LS001451	Chymotrypsin, Alpha, 3X	10gm	13930	Worthington-biochem
LS001453	Chymotrypsin, Alpha, 3X	Bulk	询价	Worthington-biochem
LS001475	Chymotrypsin, Alpha, Purified	100mg	1106	Worthington-biochem
LS001477	Chymotrypsin, Alpha, Purified	Bulk	询价	Worthington-biochem
LS001479	Chymotrypsin, Alpha, Purified	1gm	8470	Worthington-biochem
LS001628	Cholinesterase, Butyryl	500un	5040	Worthington-biochem
LS001632	Cholinesterase, Butyryl	4ku	32200	Worthington-biochem
LS001636	Cholinesterase, Butyryl	Bulk	询价	Worthington-biochem
LS001641	Clostripain (Endoproteinase-Arg-C)	1mg	630	Worthington-biochem
LS001643	Clostripain (Endoproteinase-Arg-C)	5x1mg	2436	Worthington-biochem
LS001646	Clostripain (Endoproteinase-Arg-C)	10mg	3710	Worthington-biochem
LS001647	Clostripain (Endoproteinase-Arg-C)	Bulk	询价	Worthington-biochem
LS001652	Collagen	5gm	1960	Worthington-biochem
LS001654	Collagen	1gm	616	Worthington-biochem
LS001656	Collagen	10gm	3640	Worthington-biochem
LS001658	Collagen	Bulk	询价	Worthington-biochem
LS001663	Collagen, Soluble	Bulk	询价	Worthington-biochem
LS001720	Carboxypeptidase B, PMSF Treated	Bulk	询价	Worthington-biochem
LS001722	Carboxypeptidase B, PMSF Treated	1ku	728	Worthington-biochem
LS001724	Carboxypeptidase B, PMSF Treated	3ku	1568	Worthington-biochem
LS001847	Catalase, Lyophilized	2gm	1204	Worthington-biochem
LS001849	Catalase, Lyophilized	10gm	5040	Worthington-biochem
LS001851	Catalase, Lyophilized	Bulk	询价	Worthington-biochem
LS001872	Catalase, Suspension	10ml	882	Worthington-biochem
LS001873	Catalase, Suspension	100ml	6300	Worthington-biochem
LS001874	Catalase, Suspension	Bulk	询价	Worthington-biochem
LS001896	Catalase, 0.22u Filtered	10ml	770	Worthington-biochem
LS001898	Catalase, 0.22u Filtered	10x10ml	5320	Worthington-biochem
LS002004	Deoxyribonuclease I	5mg	602	Worthington-biochem
LS002006	Deoxyribonuclease I	20mg	1470	Worthington-biochem
LS002007	Deoxyribonuclease I	100mg	5180	Worthington-biochem
LS002009	Deoxyribonuclease I	Bulk	询价	Worthington-biochem
LS002058	Deoxyribonuclease I, 0.22u Filtered	11mg	1652	Worthington-biochem
LS002060	Deoxyribonuclease I, 0.22u Filtered	25mg	3192	Worthington-biochem
LS002105	Deoxyribonucleic Acid, Calf Thymus	100mg	518	Worthington-biochem
LS002106	Deoxyribonucleic Acid, Calf Thymus	1gm	2800	Worthington-biochem
LS002107	Deoxyribonucleic Acid, Calf Thymus	5gm	11200	Worthington-biochem
LS002108	Deoxyribonucleic Acid, Calf Thymus	Bulk	询价	Worthington-biochem
LS002130	Ribonuclease A, DNase & Protease Free	Bulk	询价	Worthington-biochem
LS002131	Ribonuclease A, DNase & Protease Free	1mg	392	Worthington-biochem
LS002132	Ribonuclease A, DNase & Protease Free	5mg	1288	Worthington-biochem
LS002138	Deoxyribonuclease I	25mg	672	Worthington-biochem
LS002139	Deoxyribonuclease I	100mg	1610	Worthington-biochem
LS002140	Deoxyribonuclease I	1gm	13160	Worthington-biochem
LS002141	Deoxyribonuclease I	Bulk	询价	Worthington-biochem
LS002145	Deoxyribonuclease I	100mg	1372	Worthington-biochem
LS002147	Deoxyribonuclease I	1gm	9940	Worthington-biochem
LS002149	Deoxyribonuclease I	Bulk	询价	Worthington-biochem
LS002172	Deoxyribonuclease I, Standard Vial	5x2ku	966	Worthington-biochem
LS002173	Deoxyribonuclease I, Standard Vial	2ku	350	Worthington-biochem
LS002274	Elastase, Suspension	25mg	770	Worthington-biochem
LS002276	Elastase, Suspension	Bulk	询价	Worthington-biochem
LS002279	Elastase, Suspension	100mg	2072	Worthington-biochem
LS002280	Elastase, Suspension	1gm	15540	Worthington-biochem
LS002290	Elastase, Lyophilized	25mg	812	Worthington-biochem
LS002292	Elastase, Lyophilized	100mg	2282	Worthington-biochem
LS002294	Elastase, Lyophilized	1gm	16380	Worthington-biochem
LS002298	Elastase, Lyophilized	Bulk	询价	Worthington-biochem
LS002375	Histone, Dried	250mg	630	Worthington-biochem
LS002377	Histone, Dried	1gm	1820	Worthington-biochem
LS002379	Histone, Dried	Bulk	询价	Worthington-biochem
LS002402	Hemoglobin	5gm	518	Worthington-biochem
LS002403	Hemoglobin	25gm	1330	Worthington-biochem
LS002404	Hemoglobin	100gm	4480	Worthington-biochem
LS002407	Hemoglobin	Bulk	询价	Worthington-biochem
LS002408	Myoglobin	250mg	910	Worthington-biochem
LS002410	Myoglobin	1gm	2912	Worthington-biochem
LS002412	Myoglobin	5gm	11340	Worthington-biochem
LS002414	Myoglobin	Bulk	询价	Worthington-biochem
LS002425	Deoxyribonuclease II	80ku	1260	Worthington-biochem
LS002427	Deoxyribonuclease II	Bulk	询价	Worthington-biochem
LS002515	Hexokinase, Lyophilized, Recombinant	2.5ku	966	Worthington-biochem
LS002516	Hexokinase, Lyophilized, Recombinant	10ku	3220	Worthington-biochem
LS002518	Hexokinase, Lyophilized, Recombinant	Bulk	询价	Worthington-biochem
LS002544	Histone, Lyophilized	250mg	700	Worthington-biochem
LS002546	Histone, Lyophilized	1gm	2282	Worthington-biochem
LS002548	Histone, Lyophilized	Bulk	询价	Worthington-biochem
LS002559	Peroxidase	100mg	1190	Worthington-biochem
LS002560	Peroxidase	1gm	9310	Worthington-biochem
LS002561	Peroxidase	Bulk	询价	Worthington-biochem
LS002591	Hyaluronidase	Bulk	询价	Worthington-biochem
LS002592	Hyaluronidase	300ku	3360	Worthington-biochem
LS002594	Hyaluronidase	50ku	812	Worthington-biochem
LS002598	Cellulase, Purified	250mg	602	Worthington-biochem
LS002600	Cellulase, Purified	Bulk	询价	Worthington-biochem
LS002601	Cellulase, Purified	1gm	1442	Worthington-biochem
LS002603	Cellulase, Purified	10gm	12040	Worthington-biochem
LS002609	Cellulase	Bulk	询价	Worthington-biochem

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